New Publication in Nutrients shows beneficial effects of MicrobiomeX® in TIM-2 model

New Publication in Nutrients shows beneficial effects of MicrobiomeX® in TIM-2 model

Maastricht, The Netherlands, 17th November 2021

Press release
The effect of MicrobiomeX®, a natural citrus ingredient consisting of specific active flavonoids, was evaluated through in-vitro examination in the TIM-2 model. The study results, recently published in a peer-reviewed journal, showed that MicrobiomeX® can beneficially change the microbiota composition and Short-Chain Fatty Acids production of human volunteers.

In-Vitro Study on MicrobiomeX®

A recent in-vitro study in the TIM-2 dynamic model of the large intestine has shown that MicrobiomeX® has a profound effect on the microbiota composition, already after three days.

MicrobiomeX® is a natural Flavobiotics® from citrus fruits, designed to support gut & immune health by lowering gut inflammation and beneficially shifting the gut microbiome composition.

This innovative flavonoid-based ingredient has already shown in a first clinical trial to boost overall gut health by positively changing the short-chain fatty acid composition. In addition, lower faecal calprotectin levels (an important biomarker of gut inflammation) were found in the treatment group.

The outcome of the new in-vitro study, recently published in Nutrients, showed that the consumption of MicrobiomeX® led to a microbiota composition typical of a person following a healthy plant-based diet rich in fibre.

The study was conducted by the group of Professor Koen Venema, who is also the last author of the publication.

The purpose of the study was to find out whether low doses of MicrobiomeX® – 250mg and 350mg – would have a short term effect on the human gut microbiota and the production of short-chain fatty acids.

The study was carried out in the TIM-2 model with faecal samples from 7 human volunteers. These samples were either treated with two different doses of MicrobiomeX® or a control. Already after 48 hours, the first differences between the treatment and the control could be observed, which became significant after 72 hours.

Interestingly, a potent butyrate-producing class of bacteria – Roseburia spp – was significantly increased with both the 250mg and the 350mg doses, whereby the increases were clearly dose-dependent.

Click here to download the complete publication.

About BioActor

BioActor, based in Maastricht, Netherlands, is a product development company that has developed a range of proprietary bioactive ingredients for the nutrition & healthcare industry. The company focuses on the development of innovative activities that address active living and healthy ageing. The goal is to provide the nutrition & healthcare industry with science-based innovations that confer a real health benefit to the consumer.

Feel free to contact us for more information on the possibilities MicrobiomeX® has to offer.

Further information can also be found on:

6 Reasons to Include Actiful® in Your Energy Supplement

Top 6 Reasons to Include Actiful® in Your Energy Supplement

Top 6 Reasons to Include Actiful® in Your Energy Supplement

November 1st, 2021

As energy-boosting dietary supplements are gaining popularity amongst consumers, the need for clinically-proven health ingredients is growing accordingly. If you are a formulator looking to craft an effective product targeting energy & vitality, you may be overwhelmed by the many ingredients available out there. Can’t make your mind up? In this article, we explore the top 6 reasons to include Actiful® in your product.

6 Reasons to Include Actiful® in Your Energy Supplement

The growing interest in energy-boosting supplements.

In today’s hectic world, more than ever, people suffer from tiredness and lack of energy. Many consumers indeed tackle physical and mental stress with the help of dietary supplements, which help them with avoiding the fatigue feeling, doing more sport and being more socially active.

The interest in nutritional solutions for an extra energy kick is increasingly gaining popularity. In fact, according to the recent CRN consumer survey on dietary supplements, improving energy levels is among the top 5 reasons for consuming such products (28%, a 7% increase yearly), showing how people are relying on supplements to get through the day.

What is Actiful®?

Actiful® is a patent-protected orange and pomegranate complex specifically designed to enhance energy and vitality, affecting short-term and long-term energy systems and improving mood.

This all-natural ingredient is standardized for highly bioavailable hesperidin, an important polyphenol found in sweet orange (Citrus Sinensis). Hesperidin containing foods are part of the type of foods that are good to support your faily energy.

BioActor developed Actiful® and, together with Maastricht University, researched the ingredient and confirmed its health benefits in multiple randomized, double-blind placebo-controlled clinical trials.

Including Actiful® in your formulation: top 6 reasons

If you are thinking of including Actiful® in your formulation, you must know the following:


1. It has been clinically tested in both young and senior subjects.
When the common goal is being more active, Actiful® is the ideal ingredient for both young and senior subjects.

The efficacy of Actiful® was first demonstrated in a 4-week randomized, placebo-controlled, double-blind clinical study conducted with young subjects. The results show that immediate and sustained energy improved significantly for the supplementation group compared to placebo.

In another clinical trial, the objective was to measure the effect of Actiful® on energy and mental wellness in seniors. As expected, anaerobic power significantly improved in the supplementation group.

Interestingly, also the WHO Quality of Life scores on mental wellness significantly increased in the Actiful® group, compared to the placebo.


2. It boosts the NO levels, thereby improving the blood flow.
The active flavonoids in sweet orange, the major component of Actiful®, were found to activate the phosphorylation of Endothelial Nitric Oxide Synthase (eNOS). eNOS is the key enzyme for Nitric Oxide (NO) synthesis. Consequently, NO increases, having a beneficial effect on the endothelium by promoting vasodilation and increasing blood flow.

As a direct consequence of the improved blood flow, oxygen and nutrients are more efficiently transported to the body districts where required.

Pomegranate further augments the production of NO as it is an excellent donor of highly bioavailable nitrate.


3. It works directly at the mitochondrial level.
Experiments in myotubes showed that Hesperitin, the active flavanone derived from the metabolization of Actiful®, enhances ATP production at the mitochondrial level.

By quenching the reactive oxygen species (ROS) and reducing proton leakage between the intermembrane space and the mitochondrial matrix, Hesperitin is able to improve the proton gradient required for the ADP-ATP phosphorylation cycle.

This process results in a better “respiration capacity” of the mitochondria, leading to more ATP production, the fuel for our muscle.


4. It acts on the body as well as on the mind.
Mood is a crucial aspect of every individual’s well-being. Stress, anxiety, focus and happiness are all factors that affect our psychophysical wellness.

It was demonstrated that the supplementation with Actiful® not only positively affects the energy level, but it may also affect the mood.

As it is clear that body and mind work in tandem, Actiful® can be considered the perfect vitality enhancer!


5. High bioavailability is proven.
Bioavailability is an important aspect to consider in nutraceuticals, as it is a key indicator of absorption and directly influences the bio-activity of specific molecules.

Actiful® was developed with this concept in mind, and a human and in vitro study demonstrated that the active ingredient starts to be taken up by the body within minutes.

Furthermore, the active metabolites remain available for several hours after consumptions. Hence, Actiful® offers excellent potential for a significant immediate energy boost, maintained over time after consumption.


6. It is patent-protected.
Actiful® is an innovative and patent-protected ingredient. BioActor is a pioneer in researching the positive effect of citrus flavonoids on energy metabolism.

This further adds value and makes Actiful® even more unique. Why? Because you won’t find imitations in the marketplace.

If you are interested in learning more about Energy & Vitality – Trends, Clinical Research and Innovation, you should check out our webinar on this topic. Click here to register!